Simultaneous RP-HPLC Quantification of Praziquantel and Abamectin in Formulations: Method Development and Validation

Abstract

A validated RP-HPLC method was developed for simultaneous estimation of Praziquantel (PZ) and Abamectin (AB). Chromatographic separation was achieved using Methanol: Tetrahydrofuran: 0.1% Orthophosphoric acid in the ratio 85:05:10 (V/V/V), providing optimum peak shape and low baseline noise on an Inertsil ODS-C18 column (250 × 4.6 mm, 5μ), detected at 272 nm. Total analysis time was 10 minutes with a flow rate of 1 mL/min. Standard curves showed correlation coefficients r2r^2r2 ≥ 0.999 over concentrations 5–35 µg/mL for both drugs. The method was successfully applied for drug analysis in bulk and formulations.